Pit-Stop PCR: An Approach to Increase Final Product Yield of Multiplex PCR

Pit-stop PCR: an approach to increase final product yield of multiplex PCR.

Since the development of the polymerase chain reaction (PCR), many strategies have been designed to amplify one or more specific DNA sequences with a single set of oligonucleotide primers. Multiplex PCR is a strategy to amplify simultaneously either two or multiple DNA targets sequences in a single reaction (4). A variety of reaction conditions must be used to optimize multiplex-PCR strategies....

Real-Time PCR: an Appropriate Approach to Confi rm ssDNA Generation from PCR Product in SELEX Process

Background: Aptamers are single stranded DNA (ssDNA) or RNA molecules. The potential of aptamers for binding to the different targets has made them be widely used as the preferred diagnostic and therapeutic tools. DNA aptamers present several advantages over the RNA oligonucleotides due to their higher stability, easier selection, and production. Selection of DNA aptamers which...

The Limits of Multiplex PCR

Real-time multiplex PCR assays.

The ability to multiplex PCR by probe color and melting temperature (T(m)) greatly expands the power of real-time analysis. Simple hybridization probes with only a single fluorescent dye can be used for quantification and allele typing. Different probes are labeled with dyes that have unique emission spectra. Spectral data are collected with discrete optics or dispersed onto an array for detect...

Selecting Degenerate Multiplex PCR Primers

Single Nucleotide Polymorphism (SNP) Genotyping is an important molecular genetics technique in the early stages of producing results that will be useful in the medical field. One of the proposed methods for performing SNP Genotyping requires amplifying regions of DNA surrounding a large number of SNP loci. In order to automate a portion of this method and make the use of SNP Genotyping more wi...